app23/ps45 transgenic mice  (Novartis)

Journal: Acta Neuropathologica Communications

Article Title: MFG-E8 (LACTADHERIN): a novel marker associated with cerebral amyloid angiopathy

doi: 10.1186/s40478-021-01257-9

Figure Lengend Snippet: Study workflow for the identification of Aβ-associated proteins. a Schematic representation of the LCM proteomic approach for tissue collection, microdissection, data analysis, and protein identification. b Representative brain sections from 24-month-old APP23 and WT mice before and after LCM. I) Aβ plaques from APP23 brains stained with thioflavin S (ThS). II) Aβ-positive vessels from APP23 brains stained with ThS. III) Brain vessels from WT mice stained with tomato lectin (TL). The scale bar indicates 100 μm. c Venn diagram showing the total number of proteins identified in the three experimental groups

Article Snippet: All experiments were carried out on APP23 transgenic male mice (hemizygote B6, D2-TgN [Thy-APPSWE]-23-Tg mice, The Jackson Laboratory, Bar Harbor, ME, USA) [ ].

Techniques: Laser Capture Microdissection, Staining

Journal: Acta Neuropathologica Communications

Article Title: MFG-E8 (LACTADHERIN): a novel marker associated with cerebral amyloid angiopathy

doi: 10.1186/s40478-021-01257-9

Figure Lengend Snippet: MFG-E8 expression analysis in APP23 mice. a Representative western blot images and quantification of MFG-E8 protein levels in brain homogenates from 12-, 18-, and 24-month-old WT and APP23 mice and in 24-month-old plasma samples, n = 3–6/group. b Representative immunofluorescence images and quantification of the MFG-E8-positive vessels in brains from 24-month-old WT and APP23 mice. The scale bar indicates 100 μm, n = 3/group. c Representative confocal images showing the localization of MFG-E8 (green) and Aβ (red) in parenchymal brain sections (I) from 24-month-old APP23 mice. II) Representative images showing no MFG-E8 detection in parenchymal Aβ deposits. III) Representative images showing MFG-E8 detection in an Aβ-positive vessel. The scale bar indicates 10 μm. * p < 0.05, ** p < 0.01, **** p < 0.0001

Article Snippet: All experiments were carried out on APP23 transgenic male mice (hemizygote B6, D2-TgN [Thy-APPSWE]-23-Tg mice, The Jackson Laboratory, Bar Harbor, ME, USA) [ ].

Techniques: Expressing, Western Blot, Clinical Proteomics, Immunofluorescence